Hepatoprotective Effect of Roselle (Hibiscus sabdariffa L.) in N-Diethylnitrosamine Induced Liver Damage in Rats

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Published: Mar 6, 2023
DOI: https://doi.org/10.47191/ijmscrs/v3-i3-10

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Adam O. Abaker
Food and Nutrition Research Centre, University of Al Fashir, North Darfur State-Sudan
Abdelkareem A. Ahmed
Department of Veterinary Sciences, Faculty of Animal and Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Gaborone, Botswana.
Eman I. Kandil
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo-Egypt
Eman N. Aly
National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo-Egypt
Saber Y. Adam
Animal Welfare Center, Nyala, Sudan; Medical and Cancer Research Institute, Nyala, Sudan

Abstract

Introduction: Liver damage due N-diethylnitrosamine (DEN) is a global health challenge. The aim of this study was to assess the efficacy of roselle (Hibiscus sabdariffa L.) calyces’ water-extract as a Hepatoprotective material against liver damage induced by DEN in rats.


Material and methods: Twenty-five (25) adult female albino Wistar rats were grouped randomly into five groups (Gs): G1, Normal healthy animals (act as control); G2, DEN; G3, Roselle extract; G4, DEN + roselle-extract; G5, DEN + nanoselenium. Tissue malondialdehyde (MDA) as oxidative stress marker, and antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were measured. Also, caspase-3 as apoptotic marker and tumor necrosis factor-alpha (TNF-a) as inflammatory factor, were assessed.


Results: The results revealed decrease in MAD level and significant increase in antioxidant markers, SOD and CAT in DEN-group compared with control group. Moreover high increase in apoptotic marker, caspase-3; and significant decrease in TNF-a in DEN-group were recorded compared with normal control. Administration of roselle-extract led to significant increase in MDA and decrease in SOD and CAT parameters. In addition, high decrease in caspase-3 and increase in TNF-a was noticed. These findings were ensured by histopathological results.


Conclusion: The roselle-extract improve liver architecture, and might be involved in the hepatoprotective activity against DEN exposure. Further studies are needed for elucidation clinical applications of the plant bioactive materials and explore its mode of action in attenuating and preventing carcinogenesis.

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O. Abaker, A. ., A. Ahmed, A. ., Eman I. Kandil, N. Aly, E. ., & Y. Adam, S. (2023). Hepatoprotective Effect of Roselle (Hibiscus sabdariffa L.) in N-Diethylnitrosamine Induced Liver Damage in Rats. International Journal of Medical Science and Clinical Research Studies, 3(3), 330–336. https://doi.org/10.47191/ijmscrs/v3-i3-10
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Vol. 3 No. 3 (2023): Volume 03 Issue 03 March 2023
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References

I. Chatterjee, S. Datta, R. Dey, A., Pradhan, P., and Acharya, K. (2012). In vivo Hepatoprotective activity of Ethanolic extract of Russula albonigra against Carbon Tetrachloride-induced Hepatotoxicity in Mice. Research J. Pharm. And Tech. 5 (8): 1034-1038

II. Shanmugasundaram, P. and S. Venkataraman. (2006). Hepatoprotective and antioxidant effects of Hygrophila auriculata (K. Schum) Heine Acanthaceae root extract. Journal of Ethnopharmacology,. 104(1): p. 124-128.

III. Rumgay, H., Shield, K., Charvat, H., Ferrari, P., Sornpaisarn, B., Obot, I., et al. (2021). Global burden of cancer in 2020 attributable to alcohol consumption: a population-based study. Lancet Oncol, 22(8):1071–80. https://doi.org/10.1016/S1470-2045(21)00279-5

IV. Rumgay, H., Arnold, M., Ferlay, J., Lesi, O., Cabasag, C. J., Vignat, J., et al. (2022). Global burden of primary liver cancer in 2020 and predictions to 2040. J Hepatol., Published online 6 October 2022; https://doi.org/10.1016/j.jhep.2022.08.021

V. Plymoth, I., Chemin, P., Boffetta, P., and Hainaut, P. (2009). Hepatocellular carcinoma-a worldwide translational approach. Cancer Lett., 286:3-4.

VI. Abaker, A. O., Aly, E. N., Kandil, E. I. and Shalaby, K. F. (2015). Chemopreventive effect of Hibiscus sabdariffa calyx anthocyanins and Nano-selenium against N-diethylnitrosamine induced hepatocellular carcinoma in rats. Egy. J. Pure and App. Sci. 53 (3): 23 – 33.

VII. Sullivan, B. P., Meyer, T. J., Stershic, M. T. and Keefer, L. K. (1991). Acceleration of N-nitrosation reactions by electrophiles. IARC Sci. Pub. l: 370-374.

VIII. Reh, B. D. and Fajen, J. M. (1996). Worker exposures to nitrosamines in a rubber vehicle sealing plant. Am. Ind. Hyg. Assoc. J, 57: 918-923.

IX. Brown, J. L. (1999). N-Nitrosamines. Occup. Med. 14: 839-848.

X. Anis, K. V., Rajesh-Kumar, N. V., and Kuttan, R. (2001). Inhibition of chemical carcinogenesis by biberine in rats and mice. J. Pharm. Pharmacol. 53: 763-768.

XI. Chakraborty, T., Chatterjee, A., Rana, A., Dhachinamoorthi, D., Kumar, P. A., and Chatterjee, M. (2007). Carcinogen induced early molecular events and its implication in the initiation of chemical hepatocarcinogenesis in rats: chemopreventive role of vanadium on this process. Biochim Biophys Acta 1772:48–59.

XII. Wang, F., Qu, W., Li, Y. Liu, H., Li, B., Qi, J., Qin, C. (2016). Diethyl nitrosamine alone was not recommended for primary hepatocellular carcinoma model in rats. Int. J. Clin. Exp. Pathol, 9 (10):10346 -10350.

XIII. Abifarin, T. O., Afolayan, A. J., and Otunola, G. A. (2019). Phytochemical and Antioxidant Activities of Cucumis africanus L.f.: A Wild Vegetable of South Africa. J. Evidence-Based Integr. Med. 24: 1- 8.

XIV. Al Snafi, A. E. (2016). Medicinal plants with antioxidant and free radical scavenging effects (part 2): plant based review. IOSR J. of Pharm., 6(7): 62 – 82.

XV. Müller, B. M. and Franz, G. (1992). Chemical structure and biological activity of polysaccharides from Hibiscus sabdariffa. Planta Med., 58 :60–67. [Google Scholar] [CrossRef] [PubMed]

XVI. Mohamed, R., Fernandez, J., Pineda, M., and Aguilar, M. (2007). Roselle (Hibiscus sabdariffa) seed oil is a rich source of gamma-tocopherol. J. Food Sci. 72 :S207–S211. [Google Scholar] [CrossRef] [PubMed]

XVII. Ologundudu, A., Ologundudu, A. O., Oluba, O. M., Omotuyi, I/ O., and Obi, F. O. (2010). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits. J. Toxicol. Envir. Health Sci. 2(1): 1 – 6.

XVIII. Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M. (2014). Hibiscus sabdariffa L.-a phytochemical and pharmacological review. Food Chem. 165:424-443.

XIX. Abdel-Ghaffer, F. R. (2013) attenuation of CCl4-induced hepatic antioxidant disorder and oxidative stress by hibiscus rosasinensis extract in albino rats. Int. J. Med. Plant Altern. Med. 1(1): 1 – 12.

XX. Builders, P. F., chukuemeka, R, E., Florence, D. T. and Builders, M. I. (2010) assessment of intrinsic properties of the freeze-dried and formulated extract of Hibiscus sabdariffa Linn. (Malvaceae). Afric. J. Pharmacy and Pharmacog. 4(6): 304 – 313.

XXI. Klaunig, J. E., Pereira, M. A., Ruch, R. J. and Weghorst, C. M. (1988). Dose-response relationship of diethylnitrosamine-initiated tumors in neonatal balb/c mice: effect of phenobarbital promotion. Toxicol. Pathol. 16(3): 385 – 391.

XXII. Zhang, Z., Bi, M., Liu, Q., Yang, J., and Xu, S. (2016). Meta-analysis of the correlation between selenium and incidence of hepatocellular carcinoma. Oncotarget, 47: 77110-77116.

XXIII. Minami, M and Yoshikara, H. (1979). A simplified assay method of superoxide dismutase activity for clinical use. Clin. Chim. Acta. 92: 337 – 342

XXIV. Sinha, A. K. (1972). Colorimetric assay of catalase. Anal. Biochem. 47: 389.

XXV. Banchroft, J. D., Stevens, A. and Turner, D., R. (1996). Theory and practice of histological techniques. Fourth ed. New York.

XXVI. Former, A., Reig, M. and Bruix, J. (2018). Hepatocellular carcinoma. Lancet. 391: 1301–1314.

XXVII. Cai, Y. Z., Sun, M., Xing, J., Luo, Q., and Corke, H. (2006). Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sciences. 78: 2872–2888.

XXVIII. Ochwang, I. DO., Kimwele, C. N., Oduma, J. A., Gathumbi, P. K., Mbaria, J. M., and Kiama, S. G. (2014). Medicinal plants used in treatment and management of cancer in Kakamega County Kenya. J. Ethnopharmacol. 151:1040–1055.

XXIX. Mishra, K., Ojha, H. and Chaudhury, N. K. (2012). Estimation of antiradical properties of antioxidants using DPPH assay: a critical review and results. Food Chem, 130: 1036-1043.

XXX. Jimoh, F. O., Adedapo, A. A., and Afolayan, A. J. (2011). Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchus oleraceus. Rec. Nat. Prod. 2: 29-42.

XXXI. Lin, H. H., Chen, J. H., Kuo, W. H., Wang, C. J. (2007). Chemopreventive properties of Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation. Chem. Biol. Interact., 165: 59–75.

XXXII. Chiu, C-T., Chen, J-H., Chou, F-P. and Lin, H-H. (2015). Hibiscus sabdariffa Leaf Extract Inhibits Human Prostate Cancer Cell Invasion via Down-Regulation of Akt/NF-kB/MMP-9 Pathway, Nutrients, 7(7): 5065-5087.

XXXIII. Srivastava, A and Shivanandappa, T. (2010). Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats. Food Chem. 118: 411–417.

XXXIV. Gite, S., Yadav, S., Nilegaonkar, S., and Agte, V. (2014). Evaluation of hepatoprotective potential of functional food formulations using in vitro and in vivo models of CCl4 radical induced toxicity. Intr. J. Interdisciplinary and Multidisciplinary Studies. 1(10): 6 – 13.

XXXV. Cheng, C-S., Wang, N. and Feng, Y. (2019). Selenium in the Prevention and Treatment of Hepatocellular Carcinoma: From Biomedical Investigation to Clinical Application. Toxicology Reports, 4, (2017): 455-462