The Inflammation Spectrum of Monocytes in Relation to Obesity and Severity of Type 2 Diabetes (T2D), A Case-Control Study

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Published: Feb 8, 2024
DOI: https://doi.org/10.47191/ijmscrs/v4-i02-12
Keywords:
Monocytes; genes; T2D; inflammation; cytokines.

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Jorge Pérez-Galarza
Central University of Ecuador, Department of Molecular Biology, Faculty of Medicine, Quito, Ecuador
Lucy Baldeón R
Central University of Ecuador, Research Institute of Biomedicine, Quito, Ecuador & Central University of Ecuador, Department of Immunology, Faculty of Medicine, Quito, Ecuador
Fernando Sempértegui O
Central University of Ecuador, Department of Immunology, Faculty of Medicine, Quito, Ecuador
Behiye Özcan
Erasmus University Medical Center, Department of Internal Medicine, Rotterdam, The Netherlands
Eric JG Sijbrands
Erasmus University Medical Center, Department of Internal Medicine, Rotterdam, The Netherlands
Francisco Barrera-Guarderas
Pontifical Catholic University of Ecuador, Faculty of Medicine, Quito, Ecuador. Julio Endara Hospital, Internal Medicine Department, Ministry of Health, Quito, Ecuador
Harm de Wit
Erasmus University Medical Center, Department of Immunology, Rotterdam, The Netherlands
Anne Marie Wijkhuijs
Erasmus University Medical Center, Department of Immunology, Rotterdam, The Netherlands
Hemmo A. Drexhage
Erasmus University Medical Center, Department of Immunology, Rotterdam, The Netherlands

Abstract

Introduction. Increased monocyte and macrophage inflammatory state and pro-inflammatory cytokine production are linked to type 2 diabetes (T2D).


Research design and Methods. This is a case-control study aimed to examine the expression of 23 monocyte genes related to inflammation, adhesion, and repair in individuals with mild (mean HbA1c 7.3%, illness duration 5.6 years) and severe type 2 diabetes (mean HbA1c 8.4%, disease duration 14.2 years) compared also with lean and obese controls. In addition, we determined a set of serum inflammatory cytokines and growth factors.


Results. The monocytes of mild T2D patients (who were in general overweight/obese) showed overexpression of a subset of genes related to adhesion (CD9), vascular repair and growth (HGF). The monocytes of the severe T2D patients showed in contrast an upregulation of many of the pro-inflammatory genes, without a significantly increased expression of the repair gene HGF and the adhesion gene CD9. Serum cytokine expression in the severe T2D patients supported the increased inflammatory state of the patients showing high levels of IL-6, IL-1β, and TNF-α.


Conclusions. This study, therefore, shows a pro-inflammatory gene expression profile of monocytes of severe T2D patients, while patients with mild T2D did not show such monocyte profile.

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How to Cite
Jorge Pérez-Galarza, Lucy Baldeón R, Fernando Sempértegui O, Behiye Özcan, Eric JG Sijbrands, Francisco Barrera-Guarderas, Harm de Wit, Anne Marie Wijkhuijs, & Hemmo A. Drexhage. (2024). The Inflammation Spectrum of Monocytes in Relation to Obesity and Severity of Type 2 Diabetes (T2D), A Case-Control Study. International Journal of Medical Science and Clinical Research Studies, 4(02), 224–234. https://doi.org/10.47191/ijmscrs/v4-i02-12
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Vol. 4 No. 02 (2024): Volume 04 Issue 02 February 2024
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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