Antibacterial Potential Test of Basil Leaf (Ocimum Basilicum) Against Enterococcus Faecalis ATCC 29212
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Abstract
Introduction: Bacteria and their virulence factors are the main causes of apical periodontitis after endodontic treatment. The bacteria that is often found and considered as pathogens in root canals after endodontic treatment is Enterococcus faecalis. The main goal of endodontic treatment is the elimination of microorganisms and preventing re-infection after root canal treatment which can be achieved through mechanical-chemical debridement using an irrigation solution. Basil leaves (Ocimum basilicum) which is an herbal plant are thought to have antibacterial compounds that can inhibit Enterococcus faecalis. This research was conducted to determine the antibacterial potential of the n-hexane extract of basil leaves against Enterococcus faecalis ATCC 29212. Methods: This experimental laboratory study tested the effectiveness of n-hexane extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212 using disk diffusion method with three times of repetition. Results: The results of this study indicated that the n-hexane extract of basil leaves did not have an inhibition zone against Enterococcus faecalis ATCC 29212. Discussion: The concentration of n-hexane extract of basil leaves was not directly proportional to the diameter of the resulting inhibition zone. This is due to several factors that can affect the test results. Conclusion: The n-hexane extract of basil leaves does not have antibacterial potential effect against Enterococcus faecalis ATCC 29212.
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