The Relationship between T1DM and the Polymorphisms of IL2RA (Rs2104286) and PTPN22 (Rs2476601) Genes among Children

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Published: Sep 15, 2023
DOI: https://doi.org/10.47191/ijmscrs/v3-i9-22
Keywords:
Protein tyrosine phosphates non-receptor type 22 (PTPN22), Interleukin 2 receptor alpha (IL2RA), polymorphisms, Type 1 diabetes mellitus (T1D).

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Ashjan Saad Metroid
Ashjan Saad Metroid: Medical Laboratory Techniques, College of Health & Medical Technologies, Southern Technical University, Iraqi & Aalaa Abdulzahra Chmagh, College of medical and health technique, Southern Technical University, Iraq
Aalaa Abdulzahra Chmagh
Ashjan Saad Metroid: Medical Laboratory Techniques, College of Health & Medical Technologies, Southern Technical University, Iraqi & Aalaa Abdulzahra Chmagh, College of medical and health technique, Southern Technical University, Iraq
Khwam Reissan Hussein
Khwam Reissan Hussein, Al-Nasiriyah Technical Institute, Southern Technical University, Iraq
Mahmood Thamer Altemimi
Mahmood Thamer Altemimi, Adult Endocrinologist, Thi-Qar Specialized Diabetes Endocrine Metabolism Center, Thi-Qar Health Directorate, Thi-Qar, Iraq

Abstract

Background: Type 1 diabetes mellitus (T1DM) is an autoimmune condition caused by T lymphocytes that results in the death of pancreatic cells. Protein tyrosine phosphate non-receptor type 22 (PTPN22) and interleukin 2 receptor alpha (IL2RA) polymorphisms have been discovered to have a connection to a number of autoimmune illnesses, including T1D.


Objective: The purpose of this study is to clarify the significance of the polymorphisms PTPN22 (rs2476601) and IL2RA (rs2104286) in the susceptibility to T1DM in young Iraqi children.


Methods: The study included 60 Iraqi children diagnosed with T1DM within the past three years and a control group of 30 healthy individuals without diabetes or autoimmune diseases. In order to conduct a molecular investigation. Five ml of venous blood from 90 participants (60 patients and 30 controls) was collected for nucleic acid extraction.


Results: The PTPN22 (rs2476601) and IL2RA (rs2104286) polymorphisms were genotyped using the amplification refractory mutation system (ARMS) technique and specific primers. According to the results, PTPN22 (rs2476601) has a wild-type homozygous C/C genotype and C allele frequency of 90%, a mutant C/T genotype frequency of 10%, and no T allele. Following were the genotype frequencies for IL2RA (rs2104286): AA, AG, and GG in T1DM patients were 79%, 16%, and 4%, respectively, compared to 83%, 13%, and 3% in controls.


Conclusion: The polymorphisms of PTPN22 rs2476601 and IL2RA rs2104286 did not significantly differ in their connection with type 1 diabetes. It does not appear to affect the susceptibility of Asian Iraqis to T1D.

Article Details

How to Cite
Metroid, A. S. ., Aalaa Abdulzahra Chmagh, Khwam Reissan Hussein, & Mahmood Thamer Altemimi. (2023). The Relationship between T1DM and the Polymorphisms of IL2RA (Rs2104286) and PTPN22 (Rs2476601) Genes among Children . International Journal of Medical Science and Clinical Research Studies, 3(9), 1927–1933. https://doi.org/10.47191/ijmscrs/v3-i9-22
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Vol. 3 No. 9 (2023): Volume 03 Issue 09 September 2023
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Articles
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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